Question
Can cloning make better plants?
Answer
Cloning can indeed improve plant traits by enabling the precise manipulation and enhancement of specific genetic characteristics. This process involves the identification and cloning of genes or quantitative trait loci (QTLs) that control desirable traits, which can then be used to improve crop performance.
Key Insights on Cloning and Plant Trait Improvement
Cloning of QTLs: Cloning QTLs has been a significant method for understanding and manipulating the genetic basis of quantitative traits in plants. This approach allows for the dissection of genetic control mechanisms and can lead to the enhancement of traits such as yield, stress tolerance, and nutrient content (Salvi and Tuberosa, 2005; Salvi and Tuberosa, 2007).
Genomic Technologies: Advances in genomic technologies, such as CRISPR/Cas systems and new sequencing methods, have revolutionized plant genome engineering. These technologies facilitate the targeted improvement of plant traits by enabling precise genome modifications and the discovery of novel traits (Sedeek, Mahas and Mahfouz, 2019; Schneeberger and Weigel, 2011).
Transgenic Approaches: Transgenic technology, including the use of modular gene stacking systems, allows for the introduction of multiple favorable traits into plants. This method can enhance complex traits by modifying metabolic pathways and combining multiple genes into a single plant (Qin et al., 2022).
Artificial Selection and Clonality: Artificial selection has optimized clonality in certain crops, such as chaya, by enhancing traits like water and starch storage, which improve the plant’s fitness and propagation ability (Munguía-Rosas, 2021).
Environmental and Genetic Interactions: The improvement of plant traits can also be achieved by selecting optimal genotypes and cultivation environments. Understanding the genotype-environment interactions is crucial for enhancing traits like growth and economic yield (Deng et al., 2022).
Conclusion
Cloning and related genomic technologies offer powerful tools for improving plant traits. By enabling precise genetic modifications and the introduction of multiple favorable traits, these methods can significantly enhance crop performance, resilience, and adaptability. This potential makes cloning a valuable strategy in modern agricultural biotechnology.
References
Sedeek, K., Mahas, A., & Mahfouz, M., 2019. Plant Genome Engineering for Targeted Improvement of Crop Traits. Frontiers in Plant Science, 10. https://doi.org/10.3389/fpls.2019.00114
Salvi, S., & Tuberosa, R., 2005. To clone or not to clone plant QTLs: present and future challenges.. Trends in plant science, 10 6, pp. 297-304. https://doi.org/10.1016/J.TPLANTS.2005.04.008
Schneeberger, K., & Weigel, D., 2011. Fast-forward genetics enabled by new sequencing technologies.. Trends in plant science, 16 5, pp. 282-8. https://doi.org/10.1016/j.tplants.2011.02.006
Qin, G., Wu, S., Zhang, L., Li, Y., Liu, C., Yu, J., Deng, L., Xiao, G., & Zhang, Z., 2022. An Efficient Modular Gateway Recombinase-Based Gene Stacking System for Generating Multi-Trait Transgenic Plants. Plants, 11. https://doi.org/10.3390/plants11040488
Salvi, S., & Tuberosa, R., 2007. Cloning Qtls in Plants. **, pp. 207-225. https://doi.org/10.1007/978-1-4020-6295-7_9
Munguía-Rosas, M., 2021. Artificial selection optimizes clonality in chaya (Cnidoscolus aconitifolius). Scientific Reports, 11. https://doi.org/10.1038/s41598-021-00592-0
Deng, P., Wang, Y., Hu, F., Yu, H., Liang, Y., Zhang, H., Wang, T., Zhou, Y., & Li, Z., 2022. Phenotypic Trait Subdivision Provides New Sight Into the Directional Improvement of Eucommia ulmoides Oliver. Frontiers in Plant Science, 13. https://doi.org/10.3389/fpls.2022.832821
Goldberg, R. B., 2001. From Cot Curves to Genomics. How Gene Cloning Established New Concepts in Plant Biology. Plant Physiology: 125(1); 4-8
Jones, J. D. G, Verweij, W., Jupe, F., Cooke, D., Dorling, S., Tomlinson, L. et al, 2014.
Elevating crop disease resistance with cloned genes. Philosophical Transactions of the Royal Society: 369 (1639);
Martin, G. B., Brommonschenkel, S. H., Chunwongse, J., Frary, A., Ganal, M. W., Spivey, R. et al, 1993. Map-based cloning of a protein kinase gene conferring disease resistance in tomato
Science: 262; 1432-1432
McKinnell, R.G. and Di Berardino, M. A., 1999. Biology of Cloning: History and Rationale. BioScience: 49 (11): 875-885.
Salisbury, F. B., 2000. Biology of Cloning: History and Rationale. BioScience: 50(8); 636
Steuernagel, B., Periyannan, S. K., Hernández-Pinzón, I., Witek, K., Rouse, M. N., Yu, G., Hatta, A., Ayliffe, M., Bariana, H., Jones, J. D. G., Lagudah, E. S., Wulff, B. B. H., 2016. Rapid cloning of disease-resistance genes in plants using mutagenesis and sequence capture. Nature Biotechnology: 34; 652–655
UK Government, 2014. Marketing standards for fresh fruit and vegetables: https://www.gov.uk/guidance/comply-with-marketing-standards-for-fresh-fruit-and-vegetables [Accessed 18th November 2016]
Vleeshouwers, V. G., Raffaele, S., Vossen, J. H., Champouret, N., Oliva, R., et al, 2011. Understanding and exploiting late blight resistance in the age of effectors. Annual Reviews in Phytopathology: 49; 507–531

